Plastics – the fast facts 2023. Plastics Europe https://plasticseurope.org/knowledge-hub/plastics-the-fast-facts-2023/ (2023).
Rhodes, C. J. Solving the plastic problem: from cradle to grave, to reincarnation. Sci. Prog. 102, 218–248 (2019).
Geyer, R. in Plastic Waste and Recycling (ed. Letcher, T. M.) Ch. 2 (Academic, 2020).
Iwata, T. Biodegradable and bio-based polymers: future prospects of eco-friendly plastics. Angew. Chemie Int. Ed. 54, 3210–3215 (2015).
Bioplastics Market Development Update 2022 (European Bioplastics, 2022); https://docs.european-bioplastics.org/publications/market_data/2022/Report_Bioplastics_Market_Data_2022_short_version.pdf.
Kale, G., Auras, R. & Singh, S. P. Comparison of the degradability of poly(lactide) packages in composting and ambient exposure conditions. Packag. Technol. Sci. 20, 49–70 (2007).
Napper, I. E. & Thompson, R. C. Environmental deterioration of biodegradable, oxo-biodegradable, compostable, and conventional plastic carrier bags in the sea, soil, and open-air over a 3-year period. Environ. Sci. Technol. 53, 4775–4783 (2019).
Teixeira, S., Eblagon, K. M., Miranda, F., R. Pereira, M. F. & Figueiredo, J. L. Towards controlled degradation of poly(lactic) acid in technical applications. C 7, 42 (2021).
Datta, R. & Henry, M. Lactic acid: recent advances in products, processes and technologies – a review. J. Chem. Technol. Biotechnol. 81, 1119–1129 (2006).
Dorgan, J. R., Lehermeier, H. & Mang, M. Thermal and rheological properties of commercial-grade poly(lactic acid)s. J. Polym. Environ. 8, 1–9 (2000).
Castro-Aguirre, E., Iñiguez-Franco, F., Samsudin, H., Fang, X. & Auras, R. Poly(lactic acid)—mass production, processing, industrial applications, and end of life. Adv. Drug Deliv. Rev. 107, 333–366 (2016).
Choe, S., Kim, Y., Won, Y. & Myung, J. Bridging three gaps in biodegradable plastics: misconceptions and truths about biodegradation. Front. Chem. 9, 671750 (2021).
Tsuji, H. & Suzuyoshi, K. Environmental degradation of biodegradable polyesters 1. Poly(ε-caprolactone), poly[(R)-3-hydroxybutyrate], and and poly(L-lactide) films in controlled static seawater. Polym. Degrad. Stab. 75, 347–355 (2002).
Karamanlioglu, M. & Robson, G. D. The influence of biotic and abiotic factors on the rate of degradation of poly(lactic) acid (PLA) coupons buried in compost and soil. Polym. Degrad. Stab. 98, 2063–2071 (2013).
Siracusa, V. Microbial degradation of synthetic biopolymers waste. Polymers 11, 1066 (2019).
Williams, D. F. Enzymic hydrolysis of polylactic acid. Eng. Med. 10, 5–7 (1981).
Tokiwa, Y. & Calabia, B. P. Biodegradability and biodegradation of poly(lactide). Appl. Microbiol. Biotechnol. 72, 244–251 (2006).
Arena, M., Abbate, C., Fukushima, K. & Gennari, M. Degradation of poly (lactic acid) and nanocomposites by Bacillus licheniformis. Environ. Sci. Pollut. Res. 18, 865–870 (2011).
Prema, S. & Palempalli, U. M. D. Degradation of polylactide plastic by PLA depolymerase isolated from thermophilic Bacillus. Int. J. Curr. Microbiol. App. Sci 4, 645–654 (2015).
Zaaba, N. F. & Jaafar, M. A review on degradation mechanisms of polylactic acid: hydrolytic, photodegradative, microbial, and enzymatic degradation. Polym. Eng. Sci. 60, 2061–2075 (2020).
Pranamuda, H., Tokiwa, Y. & Tanaka, H. Polylactide degradation by an Amycolatopsis sp. Appl. Environ. Microbiol. 63, 1637–1640 (1997).
Urbanek, A. K. et al. Biochemical properties and biotechnological applications of microbial enzymes involved in the degradation of polyester-type plastics. Biochim. Biophys. Acta Proteins Proteom. 1868, 140315 (2020).
Oda, Y., Yonetsu, A., Urakami, T. & Tonomura, K. Degradation of polylactide by commercial proteases. J. Polym. Environ. 8, 29–32 (2000).
Barbier, T., Ferreira, F., Bataille, C., Dever, J. & Barbier, J. Method for preparing a polymer/biological entities blend. Patent WO2013093355 (2011).
Khan, I., Nagarjuna, R., Dutta, J. R. & Ganesan, R. Enzyme-embedded degradation of poly(ϵ-caprolactone) using lipase-derived from probiotic Lactobacillus plantarum. ACS Omega 4, 2844–2852 (2019).
Ganesh, M. & Gross, R. Embedding enzymes to control biomaterial lifetime. ACS Symp. Ser. 1043, 375–384 (2010).
Huang, Q., Hiyama, M., Kabe, T., Kimura, S. & Iwata, T. Enzymatic self-biodegradation of poly(L-lactic acid) films by embedded heat-treated and immobilized proteinase K. Biomacromolecules 21, 3301–3307 (2020).
Greene, A. F. et al. 3D-printed enzyme-embedded plastics. Biomacromolecules 22, 1999–2009 (2021).
DelRe, C. et al. Near-complete depolymerization of polyesters with nano-dispersed enzymes. Nature 592, 558–563 (2021).
Huang, Q. Y., Kimura, S. & Iwata, T. Thermal embedding of Humicola insolens cutinase: a strategy for improving polyester biodegradation in seawater. Biomacromolecules 24, 5836–5846 (2023).
Huang, Q. Y., Kimura, S. & Iwata, T. Development of self-degradable aliphatic polyesters by embedding lipases via melt extrusion. Polym. Degrad. Stab. 190, 109647 (2021).
Xu, C. et al. Investigation of the thermal stability of proteinase K for the melt processing of poly(L-lactide). Biomacromolecules 23, 4841–4850 (2022).
Sukkhum, S., Tokuyama, S. & Kitpreechavanich, V. Development of fermentation process for PLA-degrading enzyme production by a new thermophilic Actinomadura sp. T16-1. Biotechnol. Bioprocess Eng. 14, 302–306 (2009).
Sukkhum, S., Tokuyama, S., Tamura, T. & Kitpreechavanich, V. A novel poly (L-lactide) degrading actinomycetes isolated from Thai forest soil, phylogenic relationship and the enzyme characterization. J. Gen. Appl. Microbiol. 55, 459–467 (2009).
Rawlings, N. D. & Barrett, A. J. Evolutionary families of peptidases. Biochem. J. 290, 205–218 (1993).
Azrin, N. A. M., Ali, M. S. M., Rahman, R. N. Z. R. A., Oslan, S. N. & Noor, N. D. M. Versatility of subtilisin: a review on structure, characteristics, and applications. Biotechnol. Appl. Biochem. https://doi.org/10.1002/bab.2309 (2022).
Tatàno, F., Pagliaro, G., Di Giovanni, P., Floriani, E. & Mangani, F. Biowaste home composting: experimental process monitoring and quality control. Waste Manag. 38, 72–85 (2015).
Wilmouth, R. C. et al. X-ray snapshots of serine protease catalysis reveal a tetrahedral intermediate. Nat. Struct. Biol. 8, 689–694 (2001).
Hedstrom, L. Serine protease mechanism and specificity. Chem. Rev. 102, 4501–4523 (2002).
Peek, K., Daniel, R. M., Monk, C., Parker, L. & Coolbear, T. Purification and characterization of a thermostable proteinase isolated from Thermus sp. strain Rt41A. Eur. J. Biochem. 207, 1035–1044 (1992).
Guemard, E. & Dalibey, M. Liquid composition comprising biological entities and uses thereof. Patent WO2019043145 (2018).
Fukuda, N., Tsuji, H. & Ohnishi, Y. Physical properties and enzymatic hydrolysis of poly(L-lactide)-CaCO3 composites. Polym. Degrad. Stab. 78, 119–127 (2002).
Chateau, M. & Rousselle, J.-P. Masterbatch composition comprising a high concentration of biological entities. Patent WO2016198650 (2016).
Ye, G., Gu, T., Chen, B., Bi, H. & Hu, Y. Mechanical, thermal properties and shape memory behaviors of PLA/PCL/PLA-g-GMA blends. Polym. Eng. Sci. 63, 2084–2092 (2023).
Aliotta, L., Gigante, V., Geerinck, R., Coltelli, M. B. & Lazzeri, A. Micromechanical analysis and fracture mechanics of poly(lactic acid) (PLA)/polycaprolactone (PCL) binary blends. Polym. Test. 121, 107984 (2023).
Zhang, D. et al. Characterization of biodegradable food packaging films prepared with polyamide 4: influence of molecular weight and environmental humidity. Food Bioeng. 1, 276–288 (2022).
Jeong, H. et al. Mechanical properties and cytotoxicity of PLA/PCL films. Biomed. Eng. Lett. 8, 267–272 (2018).
Bher, A., Cho, Y. & Auras, R. Boosting degradation of biodegradable polymers. Macromol. Rapid Commun. 44, e2200769 (2023).
García-Depraect, O. et al. Enhancement of biogas production rate from bioplastics by alkaline pretreatment. Waste Manag. 164, 154–161 (2023).
Li, H. et al. The Sequence alignment/map format and SAMtools. Bioinformatics 25, 2078–2079 (2009).
Grabherr, M. G. et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat. Biotechnol. 29, 644–652 (2011).
Altschul, S. F. et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389–3402 (1997).
Madeira, F. et al. Search and sequence analysis tools services from EMBL-EBI in 2022. Nucleic Acids Res. 50, W276–W279 (2022).
Liu, H. & Naismith, J. H. An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol. BMC Biotechnol. 8, 91 (2008).
Studier, F. W. Protein production by auto-induction in high density shaking cultures. Protein Expr. Purif. 41, 207–234 (2005).
Tartof, K. D. Improved media for growing plasmid and cosmid clones. Bethesda Res. Lab. Focus 9, 12 (1987).
Gasteiger, E. et al. The Proteomics Protocols Handbook (Humana, 2005); https://doi.org/10.1385/1592598900.
Vonrhein, C. et al. Data processing and analysis with the autoPROC toolbox. Acta Crystallogr. Sect. D 67, 293–302 (2011).
McCoy, A. J. et al. Phaser crystallographic software. J. Appl. Crystallogr. 40, 658–674 (2007).
Langer, G., Cohen, S. X., Lamzin, V. S. & Perrakis, A. Automated macromolecular model building for X-ray crystallography using ARP/wARP version 7. Nat. Protoc. 3, 1171–1179 (2008).
Adams, P. D. et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr. Sect. D 66, 213–221 (2010).
Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr. Sect. D 66, 486–501 (2010).
Krivov, G. G., Shapovalov, M. V. & Dunbrack, R. L. Improved prediction of protein side-chain conformations with SCWRL4. Proteins 77, 778–795 (2009).
Olsson, M. H. M., SØndergaard, C. R., Rostkowski, M. & Jensen, J. H. PROPKA3: consistent treatment of internal and surface residues in empirical pKa predictions. J. Chem. Theory Comput. 7, 525–537 (2011).
Kaminski, G. A., Friesner, R. A., Tirado-rives, J. & Jorgensen, W. L. Evaluation and reparametrization of the OPLS-AA force field for proteins via comparison with accurate quantum chemical calculations on peptides. J. Phys. Chem. B 105, 6474–6487 (2001).
McAliley, J. H. & Bruce, D. A. Development of force field parameters for molecular simulation of polylactide. J. Chem. Theory Comput. 7, 3756–3767 (2011).
Jorgensen, W. L., Chandrasekhar, J., Madura, J. D., Impey, R. W. & Klein, M. L. Comparison of simple potential functions for simulating liquid water. J. Chem. Phys. 79, 926–935 (1983).
Bussi, G., Donadio, D. & Parrinello, M. Canonical sampling through velocity rescaling. J. Chem. Phys. 126, 014101 (2007).
Hess, B. P-LINCS: a parallel linear constraint solver for molecular simulation. J. Chem. Theory Comput. 4, 116–122 (2008).
Cheatham, T. E., Miller, J. L., Fox, T., Darden, T. A. & Kollman, P. A. Molecular dynamics simulations on solvated biomolecular systems: the particle mesh Ewald method leads to stable trajectories of DNA, RNA, and proteins. J. Am. Chem. Soc. 117, 4193–4194 (1995).
Kumari, R., Kumar, R. & Lynn, A. g-mmpbsa – a GROMACS tool for high-throughput MM-PBSA calculations. J. Chem. Inf. Model. 54, 1951–1962 (2014).
Daudé, D., Topham, C. M., Remaud-Siméon, M. & André, I. Probing impact of active site residue mutations on stability and activity of Neisseria polysaccharea amylosucrase. Protein Sci. 22, 1754–1765 (2013).
Henton, D. E., Gruber, P., Lunt, J. & Randall, J. in Natural Fibers, Biopolymers, and Biocomposites (eds Mohanty, A. K., Misra, M. & Drzal, L. T.) Ch. 16 (CRC, 2005).
Fischer, E. W., Sterzel, H. J. & Wegner, G. Investigation of the structure of solution grown crystals of lactide copolymers by means of chemical reactions. Kolloid-Zeitschrift Zeitschrift für Polym. 251, 980–990 (1973).
Cresson, R. et al. Etude interlaboratoires pour l’harmonisation des protocoles de mesure du potentiel bio-méthanogène des matrices solides hétérogènes (ADEME, 2014).
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